Optimization of cell culture processes for the production of. Optimization of cell culture processes for the production. Rapid, automated atline antibody titre measurements advance. Maximum oxygen transfer flux was determined at maximum cell density for these two scales. Because all of the antibodies produced by descendants of one hybridoma cell are identical, monoclonal antibod. In the present work, the common characterization phase required in monoclonal antibody production is presented by using a chos strain and bionets f0baby 2cc bioreactor. Current therapeutic antibody production and process. Cd hybridoma medium was supplemented with 8 mm lglutamine prior to use. Process schemes for monoclonal antibody production in plants one key characteristic of plantbased manufacturing processes is the broad spectrum of techniques initially used by academic and industrial development teams to facilitate extraction, clarification and product recovery fig. Scaleup of aeration and agitation strategy of a monoclonal antibody production process using a constant otf approach. Apr 21, 2020 another option that would accelerate the cgmp production would be to derive the seed culture from the expanded culture at the time the master cell bank is deposited by using half of the expanded. Many developers have attempted to increase mab output from cell culture by addressing both equipment and consumables. As the market demand for monoclonal antibodies is increasing, there is significant interest in developing proper models for mammalian cell culture processes, due to the fact that these are commonly used as production platforms for mabs, which are the fastest growing segment of the biopharmaceutical industry 16.
We investigated the capabilities of eppendorf bioblu singleuse vessels for cell culture scaleup from small to pilot scale. Application of a quality by design approach to the cell. Improving the productivity of a biopharmaceutical chinese hamster ovary cho fedbatch cell culture can enable cost savings and more efficient manufacturing capacity utilization. Schematic manufacturing process of monoclonal antibodies from cell.
It is twofold higher than is reported elsewhere for antibody production using a similar mabproducing cho cell line in an atf perfusion process 10. Several expression vectors have been optimized specifically for mab production. In comparative cell growth studies, hybridoma tp43. Cell culture process characterization requires support from the purification and analytical groups to test process impact and monitor product quality. The quality of the antibody product was not affected in the mcl. It was a dream of scientists to produce mabs for different antigens.
Cell culture processes for monoclonal antibody production ncbi. Monoclonal antibody production committee on methods of. This bioreactor has a working volume of 2l and it is equipped with the required process analytical technologies, a variable speed pump for fed batch, and an advanced gas. One method for increasing cho cell productivity is the addition of histone deacetylase hdac inhibitors to the cell culture process. Over the past three decades, the biopharmaceutical market has become a significant component of the global pharmaceutical market accounting for around 40% of its sales. In view of these difficulties, serum free media are being increasingly used for culturing hybridoma cells. However, mammalian cells have been considered difficult to work. Mammalian cell culture is the dominant expression system for therapeutic antibody production.
You receive cell culture supernatants from the best clones for your own applications. Milstein and kohler described the first technique developed for stable monoclonal antibody production in 1975. Cell culture scaleup in stirredtank singleuse bioreactors. The simplest of them is batch production, which consists of a closed system where a bioreactor is sterilized. Unlike polyclonal antibodies, which are produced in live animals, monoclonal antibodies are produced in vitro using tissueculture techniques. Animal cell culture technology has advanced significantly over the last few decades and is now generally considered a reliable, robust and. The production of complex biomedical proteins represents one of the primary applications in animal cell culture. Another option that would accelerate the cgmp production would be to derive the seed culture from the expanded culture at the time the master cell bank is deposited by using half of the expanded. After being transfected with plasmids bearing the antibody light and heavy chain genes, as well as selectable marker or markers, cells are screened for high productivity following growth recovery, serumfree suspension adaptation. All steps in the cell culture process and all process parameters in each step were identified by using a causeandeffect diagram. Cell culture process development starts with cell line generation and selection, followed by media and culture condition optimization in small. B cells from the spleen of the immunized animal are then removed. Comparing culture methods in monoclonal antibody production. Monoclonal antibodies, to act on behalf of the institute.
This case study describes the application of quality by design elements to the process of culturing chinese hamster ovary cells in the production of a monoclonal antibody. Increasing demand for monoclonal antibodies mabs useful as immunodiagnostic reagents in basic research applications and potential immunotherapeutics has created a need to optimize protein production techniques. Cell types used in these culture processes were traditionally yeasts, fungi and bacteria. The objective of cell culture media is to sustain cell growth in order to quickly seed the production bioreactor. A systematic approach to the validation of process control parameters for monoclonal antibody production in fedbatch culture of a murine myeloma. In development of all pharmaceutical production processes, including those involving hmabs produced by cho cells, decisions regarding the best process. Developing therapeutic monoclonal antibodies at pandemic. The term antibody production has both general and specific meanings. Production of monoclonal antibodies was one of the most important techniques of biotechnology to emerge during the last quarter of the 20th century. Cell culture processes in monoclonal antibody production li. Immunoconjugates are typically produced by chemical processes using specific reagents to.
Recent advances in largescale production of monoclonal. This maximum otf was kept constant for scaling up to 80 l table 1. Aggregates in monoclonal antibody manufacturing processes. The requirements of different cell lines may vary heavily resulting in the challenge of finding optimum settings for temperature and ph, gassing rates as well as nutrient composition as they influence cell growth and product titers. We also summarize the current thinking on appropriate process development strategies and process advances that might affect process development. Mammalian cell culture process for monoclonal antibody production.
Antibodies, institute for laboratory animal research, national research council. The choice of which culture mode to use for production depends on budget, time available, and the quantity of hmab required. Introduction to antibody production and purification thermo. After being transfected with plasmids bearing the antibody light and heavy chain genes, as well as selectable marker or markers, cells are screened for high productivity following growth recovery, serumfree suspension adaptation and amplification if necessary. In this study, we examined the effect of valproic acid vpa, 2propylpentanoic. In the broad sense, it refers to the entire process of creating a usable specific antibody, including steps of immunogen preparation, immunization, hybridoma creation, collection, screening, isotyping, purification, and labeling for direct use in a particular method. Imbaratto, cell culture manufacturing, covance biotechnology services, inc. Biotherapeutic production relies on robust cell lines with reproducible growth, titre and quality, so determining the titre of biopharmaceuticals at multiple points during the production process is valuable, reports kurt pickle, business line leader, bioprocess analytics group. Process schemes for monoclonal antibody production in plants one key characteristic of plantbased manufacturing processes is the broad spectrum of techniques initially used by academic and industrial development teams to facilitate extraction, clarification and product recovery.
The rapid development of highyielding and robust manufacturing processes for monoclonal antibodies is an area of significant focus in the biopharmaceutical landscape. Eb66 clones when grown in erlenmeyer flask with standard fed batch culture produces 1. The cell culture process was initially developed at 2 l and 10 l scale. Cell culture processes for monoclonal antibody production. The use of organisms as biopharmaceutical production factories offers several advantages over chemical synthesis. Some cell culture materials from characterization studies can be used as feed stream for downstream process characterization, especially for robustness studies. Animal cell culture technology has advanced significantly over the last few decades and is now generally considered a reliable, robust and relatively mature technology. Advances in mammalian cell culture have taken titers to beyond the 5gl mark. Monoclonal antibody, antibody produced artificially through genetic engineering and related techniques. Despite having the same host cell, cell growth was different between mab i and mab ii.
Guideline on development, production, characterisation and. The workshop on monoclonal antibody production was held in angera, italy, on 19. Optimization of cell culture processes for the production of therapeutic monoclonal antibodies. Aggregates in monoclonal antibody manufacturing processes marava. Cell culture processes in monoclonal antibody production. Monoclonal antibody mab is a single type of antibody that is directed against a specific antigenic determinant epitope. Guidelines to cell engineering for monoclonal antibody production. Largescale production of monoclonal antibodies monoclonal antibodies mabs and related proteins have been shown to successfully target a wide range of extra.
Polyclonal and monoclonal antibody production microbiology. Further by accumulation of monoclonal antibodies in supernatant culture no degradation was observed in antibody production assessed by hplc, sdspage and western blot. The production of monoclonal antibodies allows the isolation of reagents with a unique, chosen specificity. The cell culture for mab production can follow three different types of processes. Mammalian cell culture process for monoclonal antibody. Ptc in manufacture and testing of monoclonal antibody.
Current therapeutic antibody production and process optimization. Sep 04, 2019 rapid, automated atline antibody titre measurements advance biopharmaceutical production 4sep2019. A case study of monoclonal antibody mab production using bioblu singleuse vessels was performed at our application laboratory in enfield, ct, usa. Developing therapeutic monoclonal antibodies at pandemic pace. Rita costa a, elisa rodrigues m, henriques m, azeredo j, oliveira r eur j pharm biopharm, 742. Microbials for the production of monoclonal antibodies and. Fermentation and cell culture processes springerlink. Nov 25, 20 this chapter outlines cell culture processes in monoclonal antibody production. This technique consists of creating a hybridoma, a stable hybrid cell capable of producing a single type of antibody against a specific epitope present in an antigen. For example, recent advances in improved bioreactor. Li f1, vijayasankaran n, shen ay, kiss r, amanullah a. The doubling times measured on the three cell lines are presented in table 1. Fermentation processes may be used for the production of biomass, enzymes, chemicals and pharmaceuticals. Several of these have been scaled to largescale produc.
Lang2 1manufacturing science and technology, lonza biologics porrin. All steps in the cell culture process and all process parameters in each step were identified by. Greenfield danafarber cancer institute, boston, massachusetts 02115. Hybridoma construction was initially produced from murine models. Characterization of a monoclonal antibody cell culture. Introduction to antibody production and purification. Review aggregates in monoclonal antibody manufacturing processes mar. The cell substrate to be used for the production of the monoclonal antibodies should be a stable and continuous monoclonal cell line that has been developed by means of recombinant dna andor other. This socalled polyclonal antibody response is also typical of the response to infection by the human immune system. A range of biotherapeutics are currently synthesized using cell culture methods in large scale manufacturing facilities that produce products for both commercial use and clinical studies. Monoclonal antibody production using animal cell culture.
Immunoconjugates are typically produced by chemical processes using specific reagents to link the. Enhancing antibody production bioprocess international. Scaleup considerations for monoclonal antibody production. Downstream process development comprises cell harvest, antibody capture, viral inactivation, and polishing steps. Platform approaches to downstream process development have become widely established. The cell culture for mab production can follow three di erent types of processes. The process for development of a stable cell line starts with expression vector construction and transfection. The first monoclonal antibodies mabs have emerged from a survey of. Pdf cell culture processes in monoclonal antibody production. Grow cho cells to high density in serumfree suspension culture 700 600 500 400 300 200 100 0 0246810 12 celligen rbetagal shake flask rbetagal innovations in cell culture the largest manufacturer of cell culture media in the world. Upstream processes for monoclonal antibodies contract pharma. Cell culture recombinant mammalian cell culture is the most popular expression system to produce monoclonal antibodies, and chinese hamster ovary cho and murine myeloma ns0 are the most widely used cell lines. Rita costa a, elisa rodrigues m, henriques m, azeredo j, oliveira r eur j pharm biopharm. Production processes for monoclonal antibodies intechopen.
Trends in upstream and downstream process development. This is an excellent and easy to follow guide that will allow you to reproduce all the steps immunization, hybridoma production, screening, cloning, expanding and freezing down hybridomas, and antibody validation required for the production of a high quality monoclonal antibodies. Antiserum drawn from an animal will thus contain antibodies from multiple clones of b cells, with each b cell responding to a specific epitope on the antigen figure 2. Addition of valproic acid to cho cell fedbatch cultures. This chapter outlines cell culture processes in monoclonal antibody production. Microorganisms can produce high molecular weight compounds such as proteins. In the early years, animals were immunized against a specific antigen, blymphocytes were isolated and cultured in vitro for producing mabs. When injected into a mouse, the hybridoma cells multiply and produce.949 1163 301 489 56 478 993 923 1141 1622 595 345 246 820 660 573 1590 1513 122 438 215 359 1180 1129 1283 1435 972 779 738 565 1239 197 1088