Isolation and identification of cellulose degrading. The name is also used for any naturally occurring mixture or complex of various such enzymes, that act serially or synergistically to decompose cellulosic material. Thermostable cellulase can increase the rate of reaction, decrease amount of enzyme needed, longer halflife and decrease. Isolation and characterization of cellulase producing. Screening isolation and characterization of cellulase producing microorganisms from soil. Looking at the industrial applications and the previous research on cellulase production some of them being 310 on the production and purification of cellulase the current research work was. Isolation and characterization of cellulaseproducing. Screening of cellulase producing bacteria screening for bacterial cellulase activity in microbial isolates is typically performed on plates containing crystalline cellulose or microcrystalline cellulose such as avicel in the agar at a final concentration of 0.
Pdf microbial cellulasesproduction, applications and. The present investigation was undertaken to isolate and screen the cellulase. Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Screening and identification of cellulase producing bacteria. Fungi and bacteria isolated from soil secrete several. Isolation and screening of cellulolytic bacteria from soil. After incubation of a suitable period, a zone of clearing. Cellulase is a multienzyme,system having 3 types of. Cellulose is the most abundant component of plant biomass on the earth. Bagasse powder is used as a substrate for cellulase production employing novel thermostable yeast. In order to determine the effect of temperature on cellulase production, the selected bacterial isolate was grown in carboxymethyl cellulose and 65o c for 48 hours.
When compared to the anaerobic bacteria, aerobic bacteria can perform better cellulase production of around 90%95% of bacterial cellulose degradation. Isolation and characterization of cellulase producing bacteria from soil basavaraj i. Microorganisms of the genera trichoderma and aspergillus are thought to be cellulase producers, and crude enzymes produced by these microorganisms are commercially available for. Fungi are the main cellulase producing microorganisms, though a few bacteria and actinomycetes have also been reported to yield cellulase activity. Cellulase production from species of fungi and bacteria. Characterization of some efficient cellulase producing. Optimization of the fermentation medium for maximum cellulase production was carried out. A microscopic examination revealed that the isolated strain c 1 and c 2 was rod shaped and found to be gram positive where as c 3 strain was short rod in shape and found to be. Cellulase producing bacteria from the woodyards on kallai.
Effect of cellulaseproducing bacteria on the metabolic. Seven different bacterial strains were isolated and screened for cellulase production. Cellulosomes are protuberances on the cell wall of the bacteria, which harbor stable enzyme complexes. Microbial cellulasesproduction, applications and challenges article pdf available in journal of scientific and industrial research 6411. Optimization of cellulase production from bacteria. All the samples were tenfold diluted and pour plate and streak plate with carboxy methyl cellulose medium method were employed in order to isolate bacteria strains. The wide variety of bacteria in the environment permits screening for more efficient cellulases to help overcome current challenges in biofuel production. Patagundi and basappa basawanneppa kaliwal, year2014. The present investigation deals with the isolation, screening and characterization of cellulose producing bacteria from termite gut and animal dung, as these are good source of the cellulose digesting environment.
Cellulaseproducing bacteria were isolated from soil and identified as pseudomonas fluorescens, bacillus subtilis, e. Introduction cellulose are produced mainly in plants. Based on the morphological and biochemical characteristics, the isolates were identi. It describes the current knowledge of cellulase production by submerged fermentation and solid state fermentation, properties of cellulase and cloning and expression of cellulase gene. The cellulolytic activity was measured both qualitatively and quantitatively. When the addition of cellulaseproducing bacteria in fermenting grains was 106 cfug group 3, the average metabolic activity of bacterial communities was the highest.
Although the application of bacteria in producing cellulase is less investigated, cellulases produced by bacteria are often more e. Screening and identification of cellulase producing. Production of cellulase was analyzed by dinitrosalicylic acid dns and carboxymethyl cellulase assay methods. Ruminococcus and erwinia species strains are known as cellulaseproducing bacteria, 14 while aspergillus, penicillium, trichoderma and fusarium species are revealed to produce the enzyme in fungi. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production. Bacteria, having high growth rates as compared to fungi, have better potential for commercial cellulase production. Pdf identification of cellulaseproducing bacteria from soil in. By contrast, most anaerobic microorganisms produce large 1. In our investigation of cellulolytic bacteria, the isolates from oil palm meal were screened and identified based on the phenotypic characteristics. Microbial cellulases production, applications and challenges rajeev k sukumaran, reeta rani singhania and ashok pandey biotechnology division, regional research laboratory, industrial estate p o, trivandrum 695 019 microbial cellulases find applications in various industries and constitute a major group of the industrial enzymes. Bacteria were further identified by morphological and biochemical tests and subjected to cellulase production in 250 ml erlenmayer flask using potato waste medium for 48h of fermentation period at 35oc with agitation speed of 140rpm. Microbial cellulases production, applications and challenges. For understanding the mechanism of cellulose degradation by cellulase, it is necessary to isolate, purify and characterize this enzyme.
This enzyme has various industrial applications and now considered as major. Results and discussion cellulaseproducing bacteria were isolated from soil. Pdf on mar 25, 2015, faiz rasul and others published isolation and characterization of cellulase producing bacteria. Isolation and characterization of cellulase producing bacteria from. Screening and isolation of cellulaseproducing bacteria. Pdf this study aimed to isolate and identify bacteria that can produce cellulase enzyme from nasinuan forest, kantarawichai district.
Fungi are the main cellulaseproducing microorganisms, though a few bacteria and actinomycetes have also been recently reported to yield cellulase activity table 31. Screening and identification of cellulase producing bacteria isolated from oil palm meal saowapar khianngam1, yupa pootaengon1, taweesak techakriengkrai2, somboon tanasupawat3 1faculty of animal sciences and agricultural technology, silpakorn university, phetchaburi it campus, phetchaburi 76120, thailand. Isolation and screening of cellulase producing bacteria. Cellulases are inducible enzymes that are synthesized by a large number of microorganisms during their growth on cellulosic materials.
There has been increasing interest in cellulaseproducing bacteria because of their ability to grow fast. This is produced by several microorganisms, commonly by bacteria and fungi 1,2. Cellulase15 producing bacteria and fungi were isolated from variant sources such as agricultural wastes, soil, sediments. Cellulolytic enzymes are synthesized by a number of microorganisms. Optimization and molecular identification of novel cellulose. Screening isolation and characterization of cellulase.
Optimization of cellulase production from bacteria isolated from soil. Serial dilution was done by mixing 1gm of soil sample in 100ml of sterile distilled water and further proceeding up to 5 dilutions. Potential isolates with cellulase production were identified by grams iodine dye staining method. The isolate were tentatively identified to be bacillus species based on cultural, morphological, biochemical analysis and labelled as cb3, cb4 and cb8. Isolation, screening and identification of cellulase producing bacteria.
Screening, purification and characterization of cellulase. Isolation, screening, and identification of the cellulaseproducing bacteria the sesame seed residues were collected in sterile plastic bags and numbered. Cellulases provide a key opportunity for achieving tremendous benefits of biomass utilization wen et al. Fungal cellulase is an abundant resources,is a potential raw material for the microbial production of food, fuel etc. The prospects of cellulaseproducing bacteria for the. Pdf the prospects of cellulaseproducing bacteria for. Further partial purification of cellulase was carried out by dialysis and ammonium phosphate precipitation and also to determine molecular weight by sdspage. Isolation and characterization of thermophilic cellulase. The biochemical characterizations of the isolated strains c 1, c 2 and c 3 are presented in additional file 3. Among bacteria, bacillus species produce a number of extracellular enzyme including amylases, proteinases, and polysaccharide hydrolases mawadza et al. The residues were serially diluted using sterile distilled water and cultured on basal medium agar plates containing carboxymethylcellulose cmc as a substrate for cellulase production. While cellulose is a basic structural material of most plants, it is also produced by bacteria, principally of the genera acetobacter, sarcina ventriculi and agrobacterium. Bioethanol production with cellulase enzyme from bacillus. Presence of some species of bacteria, yeast, and fungi in sugar industry wastes has been reported in a recent study 15.
This study aimed to isolate and screen the cellulase producingbacteria from sugar industry wastes molasses and purify and characterize the cellulase produced by the isolated bacterial strains. In the terrestrial environments, it is the primary product of photosynthesis and the most abundant renewable bioresource 100 billions dry tonsyear produced in the biosphere. The optimum conditions found for cellulase production were 40c at ph 10 with glucose as carbon source and ammonium sulphate as nitrogen source, and coconut cake stimulates the production of cellulase. Hemicellulaseproducing bacteria and engineering hemicellulases. Complexed cellulase systems cellulosomes on the other hand are native to anaerobic bacteria. Cellulase enzymes are isolated from fungi present in higher termite soil. Inducible cellulase production from an organic solvent. Kaliwal department of studies in biotechnology and microbiology, karnatak university, dharwad 580 003, india corresponding author abstract introduction enzymes are delicate protein molecules necessary for life. Aerobic bacteria like thermobifida also produce all components of cellulolytic system including exo and endo glucanases 8. Bacterial cellulase usually lacks one of the three cellulase activities, that is fpase. Cellulose is commonly degraded by an enzyme called cellulase.
Bacteria, which has high growth rate as compared to fungi has good potential to be used in cellulase production ariffin et al. Thus, present study was carried out to isolate cellulase producing bacteria from different environmental samples such as cow dung, compost soil and termite gut content. Soil and waste samples molasses from colony sugar mills phalia, punjab, pakistan were used for the. The cellulase producing bacteria were isolated from industrial and agricultural areas in kerala. Isolation and molecular characterization of cellulase. While the addition of cellulaseproducing bacteria increased. Research article optimization of cellulase production from. The solid cellulosic waste can be treated efficiently. The aim of present work is to illustrate the screening and characterization of cellulytic bacteria from soil and waste molasses of sugar industry. Novel celluloseproducing bacteria including one capable of producing of a bacterial cellulose having a weightaverage degree of polymerization in terms of polystyrene of 1. However, cellulase produced by bacteria is often more effective catalyst. Bacterial isolated were grown on carboxy methyl cellulose cmc agar at various optimum conditions such as. Deciphering cow dung for cellulase producing bacteria. Microorganisms that can produce cellulase enzymes cellulolytic microorganisms can degrade cellulose.
Hemicellulose is the second most abundant renewable biomass, accounting for approximately 2535% of lignocellulosic biomass and therefore bacterial enzymes involved in its degradation have also been the focus of several hemicellulase engineering studies using either rational design or directed evolution. Phylogenetic diversity and characterization of novel and. Cellulase can also be isolated from cattle waste 15, woody biomass 16, cow manure 17 and compost 18. This study focuses on the isolation and screening of cellulaseproducing bacteria from sugar industry waste molasses and characterization by morphological and biochemical analysis. Lekh ram1, kuldeep kaur2 and sandeep sharma3 department of biotechnology, arni university, kathgarh, indora, kangra, h. Bacterial, or microbial, cellulose has different properties from plant cellulose and is characterized by high. Cellulase production by bacillus subtilis isolated from. Among bacteria, pseudomonas fluorescens is the best cellulase producer among the four followed by bacillus subtilis, e. The cellulase producing bacteria was isolated from cow dung and characterized by morphological and biochemical analysis. It can be degraded by cellulase produced by cellulolytic bacteria. In the light of this background, the present study focuses on the isolation and characterization of novel bacteria from this woodyard with potentials for producing cellulase. In the present study, the strain me271 produced the highest cmcase activity after 60 h of incubation. Isolation of cellulolytic bacteria cellulase producing bacteria were isolated from the sample by serial dilution followed by plating them on a cellulose congo red agar medium. Screening, purification and characterization of cellulase from.909 1101 1037 1089 1371 759 1177 1393 860 1103 85 932 1424 516 681 622 756 479 1473 323 1074 4 873 655 409 1001 510 963 810 468